Job Description

JOB POSTING#918111

Position: RESEARCH TECHNICIAN IV

Site: Princess Margaret Research Institute

Department: Research

Reports to: Principal Investigator

Salary Range: $36.72 to $45.89 per hour

Status: Permanent Full-time

University Health Network (UHN) is looking for an experienced professional to fill the key role of Research Technician IV on our Research Lab

Transforming lives and communities through excellence in care, discovery and learning.

The University Health Network, where \xe2\x80\x9cabove all else the needs of patients come first\xe2\x80\x9d, encompasses Toronto Rehabilitation Institute, Toronto General Hospital, Toronto Western Hospital, Princess Margaret Cancer Centre and the Michener Institute of Education at UHN. The breadth of research, the complexity of the cases treated, and the magnitude of its educational enterprise has made UHN a national and international resource for patient care, research and education. With a long tradition of groundbreaking firsts and a purpose of \xe2\x80\x9cTransforming lives and communities through excellence in care, discovery and learning\xe2\x80\x9d, the University Health Network (UHN), Canada\xe2\x80\x99s largest research teaching hospital, brings together over 16,000 employees, more than 1,200 physicians, 8,000+ students, and many volunteers. UHN is a caring, creative place where amazing people are amazing the world.

University Health Network (UHN) is a research hospital affiliated with the University of Toronto and a member of the Toronto Academic Health Science Network. The scope of research and complexity of cases at UHN have made it a national and international source for discovery, education and patient care. Research across UHN\'s five research institutes spans the full spectrum of diseases and disciplines, including cancer, cardiovascular sciences, transplantation, neural and sensory sciences, musculoskeletal health, rehabilitation sciences, and community and population health. .

The Nguyen lab at Princess Margaret Cancer Centre at University Health Network is excited to recruit a new addition to the team who is passionate about scientific research, and motivated to make an impact in the field. Our lab combines experimental work with cell lines and patient-derived tumour xenograft models with advanced computational analysis approaches to understand clonal heterogeneity in human breast cancer as it pertains to disease progression and drug resistance. Our lab collaborates with other research labs across Canada, and Europe, and we are looking for someone who will integrate well into this team. Interest in our area of research is important, but direct experience in this field is not necessary.

We are seeking a Research Technician who is passionate about cancer research to participate in cutting-edge research aimed at identifying novel immunotherapies to treat cancers.

Key Duties

• Culturing and expanding human tumor-infiltrating T cells isolated from patient tumors
• Assessing T cell tumor specificity, reactivity and phenotype using flow cytometry and other techniques
• Experience with transfecting primary murine bone marrow for a study of hematopoietic stem cells in specific murine models.
• Preparing cells for single cell RNA seq analysis and genomic DNA extraction

QUALIFICATIONS

• Masters Degree or higher in a life science related field
• At minimum, four (4) years of experience with standard biomedical research laboratory techniques.
• Strong background and working experience in tissue culture techniques is required
• Prior experience with multi-color flow cytometry, T cell functional assays, ELISA and other immunological techniques are an asset
• Effective organizational, time management and prioritization skills
• Strong work ethic and able to work independently as well a part of a team

Vaccines (COVID-19 and others) are a requirement of the job unless you have an exemption on a medical ground pursuant to the Ontario Human Rights Code.

If you are interested in making your contribution at UHN, please apply on-line. You will be asked to copy and paste as well as attach your resume and covering letter. You will also be required to complete some initial screening questions.

Posting Date: April 17, 2023 Closing Date: May 17, 2023

For current UHN employees, only those who have successfully completed their probationary period, have a good employee record along with satisfactory attendance in accordance with UHN\'s attendance management program, and possess all the required experience and qualifications should apply.

UHN thanks all applicants, however, only those selected for an interview will be contacted.

UHN is a respectful, caring, and inclusive workplace. We are committed to championing accessibility, diversity and equal opportunity and welcomes all applicants including but not limited to: all religions and ethnicities, LGBTQ2s+, BIPOC, persons with disabilities and all others who may contribute to the further diversification of ideas. Requests for accommodation can be made at any stage of the recruitment process providing the applicant has met the Bona-fide requirements for the open position. Applicants need to make their requirements known when contacted.

University Health Network